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ROBERTA DÁVILA PEREIRA DE LIMA
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Effect of indoleacetic acid on salt stress tolerance in Urochloa brizantha cultivate piatã
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Leader : JUAN CARLOS ALVAREZ PIZARRO
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MEMBRES DE LA BANQUE :
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JUAN CARLOS ALVAREZ PIZARRO
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SÉRGIO LUIZ FERREIRA DA SILVA
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AIALA VIEIRA AMORIM.
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Data: 15 févr. 2023
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Urochloa brizantha cultivar piatã is a tropical forage widely cultivated for its nutritive value and adaptability to low fertility soils. However, the growth of this forage grass is threatened when soils contain high levels of salts. Salinity alters the ionic balance and the redox state of the plant, causing alterations that contribute to the loss of plant productivity. In this sense, reliable methods are needed to mitigate the effects generated by saline stress, and the application of plant hormones is an efficient way for such purposes. The aim of this study is to evaluate the salt stress tolerance responses in U. brizantha when treated with indoleacetic acid (IAA). Initially, experiments were carried out to select an adequate concentration of the hormone (50, 200 and 800 µM) via foliar application in plants subjected to saline stress with NaCl at 100 mM. Analysis of growth by weighing and length measurements of aerial and root tissues, determination of sodium and potassium contents by flame photometry, and spectrophotometric methodologies for analysis of chlorophyll, carotenoid, proline and lipid peroxidation contents were carried out. The results showed that at lower concentrations of AIA there was a better regulation of Na+ levels, an increase in the content of photosynthetic pigments and a reduction of proline in stressed plants. Therefore, a second experiment was developed with the foliar application of AIA at 50 µM in plants subjected to saline stress with NaCl at 75 mM. In this experiment, the analyzed parameters in previous experiment were evaluated and it were included the follow analysis: relative water content, electrolyte leakage, content of hydrogen peroxide and enzyme activity of catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD). POD enzymatic activity was also evaluated by zymography. The results showed that treatment with AIA in stressed plants did not promote plant growth and stimulate enzyme activity, however it was observed a reduction in electrolyte leakage, lipid peroxidation and hydrogen peroxide, as well as a decrease in sodium levels and potassium retention in piatã grass leaves, revealing a possible participation of AIA in the regulation of ionic homeostasis. It is concluded that AIA would induce salt stress tolerance in U. brizantha by acting on processes that control sodium and potassium transport to the aerial tissues.
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RIZELLE DE OLIVEIRA BARROS
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Diversity and pathogenicity of Rhizoctonia anastomosis groups associated with alliums in Brazil
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Leader : SAMI JORGE MICHEREFF
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MEMBRES DE LA BANQUE :
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MARUZANETE PEREIRA DE MELO
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ALICE MARIA GONÇALVES SANTOS
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ELIANE MAYUMI INOKUTI
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JUAN CARLOS ALVAREZ PIZARRO
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SAMI JORGE MICHEREFF
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Data: 27 févr. 2023
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Several factors can cause losses in the production of alliums (garlic, leeks, onions, and chives), but diseases are very important. Among these, the damping-off of seedlings, basal rot of leaves and dwarfing of adult plants, caused by fungi of the genus Rhizoctonia. The identification of this fungus is based mainly on the classification into hyphal anastomosis groups (AGs). The present study was undertaken to determine which Rhizoctonia species and AGs occur in alliums production areas in Brazil using internal transcribed spacer region (ITS) sequencing of ribosomal DNA (rDNA). Garlic, leeks, onions, and chives plants with damping-off of seedlings, basal rot of leaves and dwarfing of adult plants were collected in 38 production areas located in 12 municipalities and five Brazilian states from 2018 to 2022. From symptomatic plant materials were performed isolations of the associated fungi and obtained Rhizoctonia pure cultures. Forty-nine Rhizoctonia isolates were obtained from alliums in Brazil. Genomic DNA was extracted from all Rhizoctonia isolates, and PCR amplifications from the ITS-rDNA region were made with the ITS1 and ITS4 primers. PCR products were purified and sequenced. Nucleotide sequences from Rhizoctonia reference isolates obtained from GenBank were included in the analyses, and phylogenetic relationships were analyzed by Maximum Likelihood method. The phylogenetic analysis of the ITS region allowed grouping 44 of the 49 Rhizoctonia isolates into five AGs, three of binucleate Rhizoctonia (AGA-A, AG- F, and AG-K) and two of multinucleate Rhizoctonia, specifically R. solani (AG- 4 HGI and AG-4 HGIII). Five isolates were identified as binucleate Rhizoctonia but did not cluster with any AG described. This is the first worldwide report of the association of AG-F, AG-K, and AG-4 HGI with garlic, of AG-A, AG-F, and AG-4 HGIII with leek, of AG-F with onion and AG-A and AG-F with chives. There was a predominance of AG-A (40.8% of the isolates), widely distributed in Brazilian production areas and associated with all allium species studied here. Representative isolates of the found AGs were inoculated into onions, and there was a significant difference in aggressiveness levels. This is the first detailed report on the composition of Rhizoctonia AGs associated with alliums in Brazil.
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JOSÉ WELLINGTON MACÊDO VIANA
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INHIBITORY EFFECT OF LEGUME LECTINS ON THE GROWTH OF PHYTOPATHOGENIC FUNGI
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Leader : KAMILA CAMARA CORREIA
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MEMBRES DE LA BANQUE :
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ANA PAULA OLIVEIRA DE BARROS
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CLAUDENER SOUZA TEIXEIRA
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ELIANE MAYUMI INOKUTI
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IVNA RIBEIRO SALMITO MELO
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KAMILA CAMARA CORREIA
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SAMI JORGE MICHEREFF
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Data: 13 avr. 2023
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Lectins are natural bioactive proteins found in bacteria, fungi, animals, and plants, capable of interacting with oligosaccharides, monosaccharides, and glycoconjugates through a carbohydrate recognition domain. Plant lectins, especially those from legumes, have been extensively studied because they have various biological effects, including the ability to inhibit fungal growth. Due to their fungicidal activity and biodegradability, plant lectins can be used to control diseases caused by phytopathogenic fungi. Therefore, this work sought to evaluate the inhibitory effect of lectins from Vatairea macrocarpa (VML) and Parkia platycephala (PPL) on the growth of phytopathogenic fungi Colletotrichum okinawense, C. siamense, Lasiodiplodia theobromae and Rhizoctonia solani AG-4 HGI. Therefore, the lectins were purified by affinity chromatography, lyophilized, and tested for purity by SDS-PAGE and hemagglutinating activity. To test the inhibitory effect of lectins, fungal isolates were cultured in PDA medium containing VML (1000 μg/mL) and PPL (100 and 200 μg/mL), separately. As positive controls, pencycuron and methyl thiophanate fungicides were used. Plates containing only BDA were used as controls. The growth of C. siamense (isolate CMM 4081) reduced with the use of methyl thiophanate and PPL lectin at 200 μg/mL, without differing from each other, but differing from the other treatments. In the presence of the PPL lectin at 200 μg/mL, the growth of the L. theobromae isolate CMM 2295 was lower than that observed in the control. The VML lectin effectively reduced the mycelial growth of the L. theobromae isolate CMM 4039, similar to that observed with methyl thiophanate. The VML and PPL lectins did not show inhibitory activity on the growth of C. okinawense and R. solani. The inhibitory activity of these lectins can be species-dependent regarding Colletotrichum and isolated-dependent in relation to L. theobromae. The results encourage future research with lectins aimed at obtaining formulations to control diseases caused by fungi in post-harvest plants.
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GLEDSON FERREIRA MACÊDO
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CHARACTERIZATION OF PHLEBOTOMIC FAUNA IN MUNICIPALITY OF BARBALHA, CEARÁ, BRAZIL, POSSIBLY INVOLVED IN TRANSMISSION VECTOR OF TEGUMENTARY LEISHMANIASIS AMERICANA – LTA
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Leader : CLAUDIO GLEIDISTON LIMA DA SILVA
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MEMBRES DE LA BANQUE :
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LUIS RAFAEL LEITE SAMPAIO
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CLAUDIO GLEIDISTON LIMA DA SILVA
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LAURA HEVILA INOCENCIO LEITE
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MARIA DO SOCORRO VIEIRA DOS SANTOS
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Data: 29 sept. 2023
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Leishmaniasis are vector diseases that have a major impact on the public health scenario, being present in more than 98 countries and responsible for the exposure of approximately 350 million people. Vectors play an important role in the biological cycle of the disease. Given the importance of this endemic, particularly the cutaneous form, the entomological aspects of the sandflies that transmit American Cutaneous Leishmaniasis (ACL) and the etiological agent were studied in the municipality of Barbalha – CE. During the period of January and December 2022, samples were collected from lesions of patients treated at the Medical Specialties Outpatient Clinic of the Faculty of Medicine of the Federal University of Cariri and systematic collections of sandflies were carried out in the homes and surroundings of these patients, evaluating and classifying the species of vector insects, complemented by molecular analyzes directed to the genus Leishmania spp.. The capture of sandflies was carried out using CDC (Centers for Disease Control) light traps, installed in the intra-domestic and non-domestic homes of patients diagnosed with ACL. The collected vectors were screened and subsequently classified according to sex and species. A total of 1091 sandflies were collected, 510 (46.7%) males and 581 (53.3%) females, with 11 species classified as the genus Lutzomyia spp., broken down as follows: L. longipalpis (15%), L. sallesi (0.7 %), L. lenti (6.8 %), L. sordellii (0.3 %), L. intermedia (39.1 %), L. quinquefer (1.4%), L. goiana (1.9 %), L. whitmani (30.5%), L. evandroi (0.3%), L. migonei (1.0%) and L. walqueri (3%). The data obtained allow us to conclude that the phlebotomy fauna in Barbalha is diverse and differs in some patterns from data from other regions of the Ceará state and that a complementary study to characterize the species(s) involved in transmission of ACL will complement the present essay.
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HERYKA REGINA ABRANTES DA COSTA
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Electrochemical sensor based on cerium molybdate and carbon nanotubes for evaluating the thermo-oxidative stability of azithromycin
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Leader : THIAGO MIELLE BRITO FERREIRA OLIVEIRA
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MEMBRES DE LA BANQUE :
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HEBERTY DI TARSO FERNANDES FACUNDO
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MARIA APARECIDA SANTIAGO DA SILVA
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THIAGO MIELLE BRITO FERREIRA OLIVEIRA
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Data: 4 déc. 2023
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Azithromycin (AZT) is among the most successful and highly prescribed antibiotics in the world, being used in first-line treatment for respiratory tract infections, sexually transmitted diseases, dermal infections, and other bacterial manifestations. However, when it is necessary to measure the levels of this active ingredient in complex matrices, or even when you want to study the reactivity and stability of the molecule, the number of analytical possibilities with the necessary requirements for these purposes is still limited. In this work, an innovative proposal was developed with an electrochemical sensor based on cerium molybdate (Ce2(MoO4)3) and multi-walled carbon nanotubes (MWCNT) integrated into a conductive carbon paste. Despite the high reactivity of the materials present in the resulting device (Ce2(MoO4)3-MWCNT/EPC) to analyze the oxidation of the antibiotic, experimental data and computational simulations revealed that the solubility and electroactivity of the antibiotic improve in alkaline electrolyte (pH = 8) prepared in a binary mixture of CH3OH/H2O (10:90%, v/v). The charge transfer resistance and, consequently, the sensitivity of the device also improved after the heat treatment of Ce2(MoO4)3-MWCNT/EPC, as a way to eliminate the reticular water contained in the molybdate microcrystals. There is evidence that the oxidation of the antibiotic on the electrochemical sensor occurs irreversibly, losing 2e‒ of desosamine. After optimizing the electroanalytical parameters, it was possible to detect AZT with a detection limit of 230 nm, in addition to high precision of the results, demonstrated by results deviations below 4.5%. The device showed applicability to evaluate the thermo-oxidative stability of AZT under varying conditions of temperature, ultraviolet radiation and atmospheric air saturation. The results strengthened the idea that quality control of AZT-based medicines must be carried out continuously, using non-ideal laboratory standards and closer to those observed during transport, storage and administration of the products, as a way of making a more rigorous assessment of the validity and viability of the active ingredient in formulations, as well as ensuring the health of consumers.
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ALINE MARIA BRITO LUCAS
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EFFECTS OF CALORIC RESTRICTION ON CARDIAC, MITOCHONDRIAL FUNCTION AND THE REDOX STATE DURING ISOPROTERENOL-INDUCED CARDIAC HYPERTROPHY IN VIVO.
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Leader : HEBERTY DI TARSO FERNANDES FACUNDO
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MEMBRES DE LA BANQUE :
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ANA CATARINA REZENDE LEITE
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FRANCISCO NASCIMENTO PEREIRA JUNIOR
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HEBERTY DI TARSO FERNANDES FACUNDO
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IRWIN ROSE ALENCAR DE MENEZES
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ROGERIO DE AQUINO SARAIVA
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THIAGO MIELLE BRITO FERREIRA OLIVEIRA
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Data: 7 juil. 2023
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Cardiac hypertrophy is characterized by an increase in heart size due to intracellular stimuli acquired from pressure or volume overload. Although it is an adaptive and transient response, it often evolves into congestive heart failure, causing heart failure. Mitochondrial dysfunctions are involved in the development of cardiac pathologies and are generally associated with defects in the electron transport chain, Ca2+ overload, reactivation of cardiac genes and, mainly, increased oxidative stress. On the other hand, calorie restriction (CR) is a nutritional intervention that protects against cardiac hypertrophy by decreasing oxidative stress and improving mitochondrial function. In this study, it will be shown that this dietary intervention prevents the elevation of cardiac proteins, avoids the reprogramming of the atrial natriuretic factor and blocks the increase in the index of heart weight per length of the tibia (mg/cm), parameters observed in hypertrophic hearts. These results suggest that CR inhibits pathological cardiac growth while reducing mitochondrial reverse electron transport-induced hydrogen peroxide formation and improving mitochondrial content. Additionally, RC attenuated Ca2+-induced mPTP opening in mitochondria isolated from mice treated
with isoproterenol. In addition, CR blocked the negative correlation of antioxidant enzymes (superoxide dismutase and glutathione peroxidase activity), leading to the maintenance of sulfhydryl and glutathione protein levels. Given the nature of isoproterenol-induced cardiac hypertrophy, we investigated whether CR could alter cardiac β-adrenergic sensitivity. Thus, using isolated rat hearts in a Langendorff system, it was seen that rats with CR (similar to controls) preserved β-adrenergic signaling. In contrast, hypertrophic rat hearts (treated for seven days with isoproterenol) were insensitive to β-adrenergic activation with isoproterenol (50 nM). These results indicate that CR has beneficial effects against hypertrophic cardiomyopathy and may contribute to the prevention and/or treatment of complications related to cardiac hypertrophy and other cardiovascular diseases.
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VANESSA ERIKA ABRANTES COUTINHO
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LECTINS FROM Ganoderma applanatum AND Canavalia ensiformis AS ELEMENTS IN ELECTROCHEMICAL BIORECOGNITION FOR GLUCOSE
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Leader : THIAGO MIELLE BRITO FERREIRA OLIVEIRA
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MEMBRES DE LA BANQUE :
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FRANCISCO ADRIANO DE OLIVEIRA CARVALHO
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FRANCISCO NASCIMENTO PEREIRA JUNIOR
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HEBERTY DI TARSO FERNANDES FACUNDO
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TATIANE SANTI GADELHA
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THIAGO MIELLE BRITO FERREIRA OLIVEIRA
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Data: 7 juil. 2023
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Carbohydrates are the most abundant organic molecules on the planet, being important in mechanisms of intercellular recognition and interaction and for health. Different ways of controlling glucose levels have been the subject of much research aimed at facilitating and/or improving the detection of this monosaccharide in biological fluids, foods and commercial formulations. Due to their ability to reversibly and specifically bind carbohydrates, lectins are proteins that have been gaining ground as a recognition star in the development of electrochemical glucose biosensors. Legume lectins are widely studied and characterized, with the basic lectin from Canavalia ensiformes (ConA) being the best-known of this group. Due to the diversity of properties according to the source of production, fungal lectins also show biotechnological potential. The species Ganoderma applanatum, for example, has a lectin (GAL) with an affinity for glucose and which is moderately resistant to variations in pH (4 - 8) and temperature (20 – 60 ºC), although with a hemagglutinating activity that is better preserved under physiological conditions. In this work, two biorecognition systems for glucose were developed with ConA and GAL, using a glassy carbon electrode (GCE) modified with hexacyanometalate Prussian Blue (PB) which, in addition to being a semiconductor material, has heterogeneous electron density regions and suitable for the adsorption of proteins, through intermolecular bonds. The results obtained by different techniques proved that the problem of PB instability at physiological pH can be solved by thermal treatment at 100 ºC for 1h, leading to the electrode support of ta-PB/GCE. The use of artificial intelligence associated with electrochemical parameters allowed structural studies and the collection of data on biocompatible and electrostatically favorable conditions for immobilization of GAL in ta-PB/GCE, which were also useful in studies with ConA. The GAL/ta-PB/GCE biosensor showed excellent electrochemical performance for glucose quantification under ideal conditions, reaching a detection limit of 10.2 pM and sensitivity of 0.012 µA µM ‒1 cm ‒2 . The performance obtained with ConA/ta-PB/GCE was satisfactory, reaching a detection limit of 0.96 µM and sensitivity of 3.94×10 3 µA µM ‒1 cm ‒2 . The selectivity of the assays was influenced by other carbohydrates, including fructose, maltose and sucrose, probably due to the moderately low regioselectivity achieved with surface interaction sites of these proteins, but without compromising the viability of the devices for the electroanalysis of glucose in complex samples. Both biosensors were tested for glucose quantification in pharmaceutical formulations, achieving recovery values between 98.0% and
100.5%. The ConA/ta-PB/GCE biosensor was successfully applied for the analysis of total sugars in a soft drink sample. Each device presented particular advantages and challenges that still require further studies, but the set of figures of merit obtained in each system proved that the platforms developed are truly efficient for the analysis of glucose in different samples, stimulating further research with the development of electrochemical biosensors based on lectins for carbohydrates of biological relevance.
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CICERA ÉDNA BARBOSA DAVID
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INVESTIGATION OF THE EFFECTS OF CALORIC RESTRICTION ON OXIDATIVE STRESS, MITOCHONDRIAL FUNCTION AND LIPIDOMA DURING ISOPROTERENOL-INDUCED CARDIAC HYPERTROPHY IN MICE
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Leader : HEBERTY DI TARSO FERNANDES FACUNDO
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MEMBRES DE LA BANQUE :
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JANAÍNA ESMERALDO ROCHA
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ANNA LIDIA NUNES VARELA
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FRANCISCO ASSIS BEZERRA DA CUNHA
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FRANCISCO NASCIMENTO PEREIRA JUNIOR
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HEBERTY DI TARSO FERNANDES FACUNDO
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Data: 27 juil. 2023
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Cardiac hypertrophy is a compensatory response of the heart to an increase in volume or blood pressure. Although initially a beneficial response, it often leads to hypertrophic cardiomyopathy and heart failure. Studies indicate that cardiac hypertrophy induces a metabolic shift leading to a preferential consumption of glucose in relation to fatty acids. Caloric restriction, a dietary procedure that promotes health benefits and prolongs life expectancy in many organisms, produces effects involving improvement in energy metabolism and oxidative stress, in which mitochondria play a central role. In this work, the hypothesis was raised that part of the beneficial effects of caloric restriction is due to changes in lipid content, maintenance of mitochondrial and redox functions, and that together, these effects would induce the prevention of cardiac hypertrophy. In this work, the hypothesis was raised that part of the beneficial effects of caloric restriction is due to changes in lipid content, maintenance of mitochondrial and redox functions, and that together, these effects would induce the prevention of cardiac hypertrophy. To investigate these effects, a protocol for the induction of caloric restriction in mice was performed based on the average consumption of the standard diet of mice fed ad libitum for 2 weeks. The animals were then randomly divided into two groups: ad libitum intake (AL) and fed 60% of the mean calorie intake (CR) for an additional 4 weeks. Cardiac hypertrophy was induced by isoproterenol (ISO) (30 mg/kg/day) for 8 days. Mice treated with ISO had an increased heart weight/tibial length ratio and increased cardiomyocyte cross-sectional area. These hypertrophic markers were significantly reduced in ISO-treated CR mice. Mitochondria isolated from hypertrophic hearts produced significantly higher levels of H2O2 (when succinate was used without the presence of rotenone). This effect was blocked by CR. Concomitantly, cardiac hypertrophy inhibited the activity of enzymes, MnSOD, GPx, and GST, reduced rates of mitochondrial respiratory control and decreased the ATPase activity. These effects were also avoided by CR. To obtain information on how CR can interfere with hypertrophy-induced metabolic changes, cardiac lipidome was also studied. CR protected against changes in several triglycerides containing saturated fatty acids observed in hypertrophic samples. Cardiac hypertrophy induced an increase in ceramides, phosphoethanolamines and acylcarnitines (12:0, 14:0, 16:0 and 18:0), and promoted mitochondrial damage observed by swelling secondary to calcium absorption. These changes were prevented by caloric restriction. Together, these data demonstrate that hypertrophy promotes oxidative stress, mitochondrial disorders, and alters cardiac lipidome. These changes are prevented (at least partially) by in vivo caloric restriction intervention. These findings could have promising implications for the development of new treatment strategies to protect the heart against hypertrophy and its complications. However, more research is needed to deepen our understanding of the exact mechanisms by which calorie restriction exerts its beneficial effects on the heart.
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