Banca de QUALIFICAÇÃO: OLÍVIA CAROLINE MAIA DE MOURA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : OLÍVIA CAROLINE MAIA DE MOURA
DATE: 01/12/2023
TIME: 15:00
LOCAL: Google Meet
TITLE:

Evaluation of the Antibacterial Activity of Chalcone Compound and Its Action in Reversing Resistance by Efflux Pump Inhibition in Staphylococcus Aureus Strains

KEY WORDS:

Keywords: Bacterial resistance, Staphylococcus aureus, Chalcones, Efflux pump.

PAGES: 35
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The overuse of antibiotics has led to the emergence and spread of resistant microorganisms, representing a serious global health problem and a challenge for researchers around the world. In addition, in recent years, the development of new drugs capable of dealing with this growing threat has occured slowly and insufficiently. Among the most important resistant microorganisms is Staphylococcus aureus, a Gram-positive bacterium responsible for causing a wide range of infections. The high capacity of this pathogen to acquire and accumulate resistance mechanisms makes it particularly worrisome. Efflux pumps are known mechanisms that mediate the resistance of S. aureus to several classes of antibiotics. Thus, several studies have been carried out in order to find new therapeutic alternatives with antibacterial activity and that are capable of inactivating this resistance mechanism. In this context, chalcones show promise compounds, due to their ease of synthesis, wide range of activities and few toxic side effects. The aim of this study was to evaluate the antibacterial activity of nitrochalcone CAP as well as its action in the reversal resistance through the inhibition of the efflux pump in strains of Staphylococcus aureus. The determination of the Minimum Inhibitory Concentration (MIC) was carried out using the broth microdilution methodology and the efficacy of the compound was proven by reducing the MIC of the antibiotics ciprofloxacin and ampicillin when associated with the compound. Ethidium bromide was used as marker for the efflux pump tests. The results obtained in this study demonstrated that chalcone CAP was ineffective in inhibiting efflux pumps in all strains tested. However, when associated with the ampicillin, it was possible to observe that chalcone significantly potentiated the activity of the antibiotic. These results suggest that the compound has potential as a possible adjuvant to ampicillin.

COMMITTEE MEMBERS:
Externo à Instituição - THIAGO SAMPAIO DE FREITAS
Interno - FRANCISCO NASCIMENTO PEREIRA JUNIOR
Externa à Instituição - JANAÍNA ESMERALDO ROCHA
Interna - KAMILA CAMARA CORREIA