Banca de DEFESA: OLÍVIA CAROLINE MAIA DE MOURA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : OLÍVIA CAROLINE MAIA DE MOURA
DATE: 12/12/2302
TIME: 09:00
LOCAL: CCAB
TITLE:

EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF CHALCONE (2E) -3-({4-[(2E) -3-(4-NITROPHENIL) PROP-2-ENOL] PHENYL} CARBAMOYL) PROP-2-ENOIC ACID AND ITS ACTION AGAINST STAIN OF Staphylococcus aureus CARRYING EFFLOW PUMP

KEY WORDS:

Bacterial resistance, Chalcone CAP, SA-K2068, MIC, MepA.

PAGES: 41
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The emergence and spread of resistant microorganisms have raised concerns of a looming global infectious disease crisis. In addition, in recent years the increase in resistant microorganisms has been greater than the introduction of new therapeutic alternatives capable of combating them. Among the most important resistant microorganisms is Staphylococcus aureus, a Gram-positive bacterium responsible for causing an increase in infections. The high capacity of this pathogen to acquire and accumulate resistance mechanisms makes it particularly worrisome. Efflux pumps are known mechanisms that mediate the resistance of S. aureus to several classes of antibiotics. Thus, several studies have been carried out in order to find new therapeutic alternatives with antibacterial activity and that are capable of inactivating this resistance mechanism. In this context, chalcones show promise compounds, due to their ease of synthesis, a large number of biological activities and few toxic side effects. The aim of this study was to evaluate the antibacterial activity of chalcone (2E)-3-({4-[(2E)-3-(4-nitrophenil)prop-2-enol]phenyl}carbamoyl)prop-2-enoic acid, called CAP, as well  as its action on Staphylococcus aureus K2068 strain carrying the MepA efflux pump. The determination of the Minimum Inhibitory Concentration (MIC) was performed through the broth microdilution methodology and the analysis of chalcone on the efflux pump was performed using ethidium bromide as a maker. These results obtained in this study showed that chalcone CAP was not able to potentiate the action of the antibiotic ciprofloxacin and was not able to inhibit the efflux pump.

COMMITTEE MEMBERS:
Presidente - FRANCISCO NASCIMENTO PEREIRA JUNIOR
Externa à Instituição - JANAÍNA ESMERALDO ROCHA
Interna - KAMILA CAMARA CORREIA