Banca de QUALIFICAÇÃO: JULIO CESAR SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JULIO CESAR SILVA
DATE: 28/02/2024
TIME: 09:00
LOCAL: Google meet
TITLE:

FTIR analysis and antimicrobial activity of molybdic acid, potassium molybdate, calcium tungstate and sodium tungstate

KEY WORDS:

ATR-FTIR; Inorganic compounds; Molybdates; Bacterial resistance Tungstates.

PAGES: 87
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The discovery of antibiotics was one of the greatest medicinal revolutions of the 20th century. Its introduction into clinical practice allowed the control of bacterial infections, mitigating their relative contribution to global mortality rates. However, simultaneously, bacterial pathogens progressed towards resistance, in proportion to the adherence to antibiotics, which has intensified in recent years. For a medicine to be promising in replacing the effect of an antibiotic, it must necessarily have a well-defined structure. Polyoxometalates (POM’s) represent a significant class of inorganic compounds with a diversity of functions, sizes and chemical composition, characteristics that have been targeted in recent years. The objective of this study was to carry out a bibliometric and integrative survey, which included information regarding the antibacterial activity of POM's, as well as the in vitro analysis of their microbiological activity. This research was divided into two stages, in the first one a search was carried out in the Scopus and Web of Sciences databases, using the descriptors “Polyoxometalates” and “Polyoxometalates and antibacterial activity”. The chemical characterization of the compounds occurred using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Using serial microdilution methodology, with colorimetric development, microbiological studies investigated the direct effect of the compounds on the clinically important bacteria Escherichia coli and Staphylococcus aureus, and the subinhibitory effect combined with traditional antibiotics was also verified. To evaluate the inhibition of beta-lactamase activity, the ability of the compounds to reduce the MIC of ampicillin against multiresistant strains of Staphylococcus aureus was verified. Molybdates and tungstates did not present direct antibacterial activity, however they potentiated the effect of the traditional antibiotics ampicillin, ciprofloxacin and gentamicin, in different circumstances against the bacteria Escherichia coli and Staphyloccocus aureus. Both molybdic acid and potassium molybdate inhibited the resistance effect presented by the bacteria S. aureus-K4100 and K4414. Another highlight is associated with the activity of calcium tungstate, which presented an effect similar to that described for the molybdates tested. Given the current resistance scenario, these results represent new alternatives for the treatment of bacterial infections, allowing a better understanding of the properties of POMs. In terms of publication, the results presented here are unprecedented, as far as has been consulted in the literature.

COMMITTEE MEMBERS:
Presidente - FRANCISCO NASCIMENTO PEREIRA JUNIOR
Externa à Instituição - JANAÍNA ESMERALDO ROCHA
Externa à Instituição - MARIA KAROLLYNA DO NASCIMENTO SILVA LEANDRO
Externo à Instituição - THIAGO SAMPAIO DE FREITAS